September 2025

Draw a schematic diagram of spectrophotometer and enlist the basic components of it.

Introduction A spectrophotometer is an instrument used in biochemistry laboratories to measure how much light a substance absorbs. It is widely used in protein estimation, DNA quantification, and enzyme activity studies. Spectrophotometry is based on the principle that different substances absorb light at different wavelengths, and this absorbance can be used to determine their concentration. […]

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Define the molarity. How would you prepare 100 mL solution of 0.5 M NaOH.

Introduction In chemistry and biochemistry, concentration is an important concept that describes how much solute is present in a given volume of solution. One of the most common ways to express concentration is using molarity. Molarity is used in many laboratory experiments for preparing chemical solutions in precise quantities. Definition of Molarity Molarity (M) is

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Describe the different types of pipettes and their use.

Introduction Pipettes are essential tools in any biochemistry or biology laboratory. They are used to measure and transfer precise volumes of liquids, from a few microliters to several milliliters. The correct use of pipettes ensures accuracy and reproducibility in experimental work. There are different types of pipettes available, each with unique features and applications. This

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Explain why glassware cleaning is important in biochemistry laboratory.

Introduction In a biochemistry laboratory, clean glassware is absolutely essential. Whether conducting simple experiments or advanced biochemical analyses, the cleanliness of glassware can greatly affect the accuracy and reliability of the results. Contaminated or poorly cleaned glassware can introduce errors, interfere with chemical reactions, and even lead to the failure of entire experiments. Importance of

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Discuss the principle and basic points of Good Laboratory Practice (GLP)

Introduction Good Laboratory Practice (GLP) is a set of principles designed to ensure the quality, integrity, and reliability of laboratory studies. It is especially important in scientific research and biochemistry laboratories where accurate results are crucial. GLP is a quality control system that applies to both non-clinical research and product development processes. Following GLP ensures

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BBCET-151 Plant Biochemistry Assignment Answers 2025

BBCET-151 Plant Biochemistry Assignment Answers (2025) Below are the answers to all questions from the IGNOU assignment for the course BBCET-151: Plant Biochemistry for the academic year 2025. Explain the structure and function of plant cell wall. List the diverse roles of plant vacuoles. Explain Emerson enhancement effect. Describe the role of PS I (NADPH

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Write a note on biotic stress.

Introduction Biotic stress refers to the negative impact on plants caused by living organisms such as bacteria, fungi, viruses, insects, nematodes, and weeds. These stressors interfere with normal plant growth, development, and productivity. Unlike abiotic stress (caused by non-living factors like drought or temperature), biotic stress is triggered by biological entities that compete for nutrients,

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Describe organ and cell suspension culture.

Introduction Plant tissue culture is a method used to grow plant cells, tissues, or organs under sterile conditions on a nutrient medium. Two important techniques within this field are organ culture and cell suspension culture. These methods are widely used in research, biotechnology, and agriculture for cloning plants, conserving rare species, and producing valuable compounds.

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Describe role of phenolics in plants.

Introduction Phenolics, also known as phenolic compounds or polyphenols, are a large group of secondary metabolites produced by plants. These compounds contain one or more aromatic rings with hydroxyl groups and play diverse roles in plant growth, development, and defense. Phenolics are not directly involved in primary metabolism like respiration or photosynthesis, but they are

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