September 2025

Illustrate the application of genetic engineering in medicine, focusing on the production of recombinant human insulin.

Introduction Genetic engineering has transformed the field of medicine by enabling the development of new drugs, therapies, and diagnostic tools. One of the most successful applications of genetic engineering is the production of recombinant human insulin. In this answer, we’ll explore how genetic engineering is applied in medicine, with a focus on the process of […]

Illustrate the application of genetic engineering in medicine, focusing on the production of recombinant human insulin. Read More »

Discuss the principles and methods used in DNA sequencing. Why is sequencing essential in genetic studies?

Introduction DNA sequencing is the process of determining the exact order of nucleotides (A, T, C, G) in a DNA molecule. It plays a critical role in understanding genetics, diagnosing diseases, and advancing biotechnology. In this answer, we will discuss the principles and methods of DNA sequencing and explain its importance in genetic studies. Principles

Discuss the principles and methods used in DNA sequencing. Why is sequencing essential in genetic studies? Read More »

Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to genetic engineering?

Introduction The Polymerase Chain Reaction (PCR) is one of the most powerful techniques in molecular biology. It allows scientists to make millions of copies of a specific DNA segment in just a few hours. PCR has many applications in research, medicine, and genetic engineering. In this answer, we will describe the PCR process, explore its

Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to genetic engineering? Read More »

Explain the significance of synthetic oligonucleotides in molecular cloning and the steps involved in their synthesis.

Introduction In molecular cloning, synthetic oligonucleotides play a vital role. These short sequences of nucleotides are artificially made in the lab and are used in a wide range of genetic techniques, including gene cloning, PCR, and DNA sequencing. In this answer, we will discuss the importance of synthetic oligonucleotides and explain the steps involved in

Explain the significance of synthetic oligonucleotides in molecular cloning and the steps involved in their synthesis. Read More »

What are the steps involved in gene cloning, and why is a cloning vector necessary?

Introduction Gene cloning is a fundamental process in molecular biology and genetic engineering. It involves making multiple copies of a specific gene or DNA segment. This technique is widely used in medicine, agriculture, and research. In this answer, we will explain the main steps of gene cloning and discuss the important role of a cloning

What are the steps involved in gene cloning, and why is a cloning vector necessary? Read More »

Explain the mechanism of action of DNA ligase in joining sticky and blunt-ended DNA fragments.

Introduction DNA ligase is a key enzyme in molecular biology and genetic engineering. It acts as a molecular glue that joins DNA fragments together. Whether DNA has sticky ends or blunt ends, DNA ligase plays a crucial role in sealing the gaps and forming a stable DNA molecule. In this answer, we will explain how

Explain the mechanism of action of DNA ligase in joining sticky and blunt-ended DNA fragments. Read More »

Compare and contrast Southern, Northern, and Western blotting techniques. What are their applications?

Introduction Blotting techniques are essential tools in molecular biology used to detect specific molecules like DNA, RNA, or proteins. There are three main types of blotting methods: Southern blotting, Northern blotting, and Western blotting. Although they share similar basic steps, each technique targets a different molecule. In this answer, we will compare and contrast these

Compare and contrast Southern, Northern, and Western blotting techniques. What are their applications? Read More »

What is the role of restriction endonucleases in genetic engineering? List and classify their types.

Introduction Restriction endonucleases, also known as restriction enzymes, are essential tools in genetic engineering. They act like molecular scissors, cutting DNA at specific sites. This precise cutting allows scientists to isolate genes, insert them into vectors, and carry out recombinant DNA technology. In this answer, we’ll explore the role of restriction endonucleases in genetic engineering,

What is the role of restriction endonucleases in genetic engineering? List and classify their types. Read More »

Define Recombinant DNA Technology and describe the steps involved in its process.

Introduction Recombinant DNA technology is a revolutionary technique in molecular biology that allows scientists to combine DNA from different organisms. This has paved the way for major advancements in medicine, agriculture, and biotechnology. In this answer, we will define recombinant DNA technology and explain the steps involved in its process in simple and clear terms.

Define Recombinant DNA Technology and describe the steps involved in its process. Read More »

BBCCT-123 Assignment Answer Links – Gene Expression and Regulation (2025)

Assignment Overview This post provides direct links to the answers for the BBCCT-123: Gene Expression and Regulation assignment for the year 2025. Each question has been answered in detail using simple, student-friendly language (suitable for 10th standard level and above). Click on the links below to view individual answers. Answer Links A) Define transcription and

BBCCT-123 Assignment Answer Links – Gene Expression and Regulation (2025) Read More »

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