September 2025

Compare and contrast Southern, Northern, and Western blotting techniques. What are their applications?

Introduction Blotting techniques are essential tools in molecular biology used to detect specific molecules like DNA, RNA, or proteins. There are three main types of blotting methods: Southern blotting, Northern blotting, and Western blotting. Although they share similar basic steps, each technique targets a different molecule. In this answer, we will compare and contrast these […]

Compare and contrast Southern, Northern, and Western blotting techniques. What are their applications? Read More »

What is the role of restriction endonucleases in genetic engineering? List and classify their types.

Introduction Restriction endonucleases, also known as restriction enzymes, are essential tools in genetic engineering. They act like molecular scissors, cutting DNA at specific sites. This precise cutting allows scientists to isolate genes, insert them into vectors, and carry out recombinant DNA technology. In this answer, we’ll explore the role of restriction endonucleases in genetic engineering,

What is the role of restriction endonucleases in genetic engineering? List and classify their types. Read More »

Define Recombinant DNA Technology and describe the steps involved in its process.

Introduction Recombinant DNA technology is a revolutionary technique in molecular biology that allows scientists to combine DNA from different organisms. This has paved the way for major advancements in medicine, agriculture, and biotechnology. In this answer, we will define recombinant DNA technology and explain the steps involved in its process in simple and clear terms.

Define Recombinant DNA Technology and describe the steps involved in its process. Read More »

BBCCT-123 Assignment Answer Links – Gene Expression and Regulation (2025)

Assignment Overview This post provides direct links to the answers for the BBCCT-123: Gene Expression and Regulation assignment for the year 2025. Each question has been answered in detail using simple, student-friendly language (suitable for 10th standard level and above). Click on the links below to view individual answers. Answer Links A) Define transcription and

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How are antibiotics used to inhibit transcription in prokaryotes? Provide examples of such inhibitors.

Introduction Antibiotics are medicines used to kill or stop the growth of bacteria. One way antibiotics work is by targeting bacterial transcription – the process by which bacteria make RNA from DNA. In this answer, we’ll explore how antibiotics inhibit transcription in prokaryotes and provide examples of commonly used antibiotics that act this way. What

How are antibiotics used to inhibit transcription in prokaryotes? Provide examples of such inhibitors. Read More »

A) How do environmental conditions influence gene expression in prokaryotes? Provide examples.

Introduction Prokaryotic organisms, such as bacteria, live in constantly changing environments. To survive, they must be able to quickly turn genes on or off in response to different conditions. This process is known as gene regulation. In this answer, we’ll explore how environmental conditions influence gene expression in prokaryotes, with the help of simple examples.

A) How do environmental conditions influence gene expression in prokaryotes? Provide examples. Read More »

Explain chromatin remodeling and its role in gene expression in eukaryotes.

Introduction In eukaryotic cells, DNA is not free-floating but is tightly packed into a structure called chromatin. For genes to be expressed, the DNA must be accessible to the cell’s machinery. This is where chromatin remodeling comes into play. In this answer, we’ll explain what chromatin remodeling is and how it influences gene expression in

Explain chromatin remodeling and its role in gene expression in eukaryotes. Read More »

Discuss the operon concept and its significance in prokaryotic gene regulation.

Introduction Gene regulation is important for controlling when and how genes are turned on or off in a cell. In prokaryotic organisms like bacteria, a unique system called the operon helps manage this process efficiently. In this explanation, we will discuss the operon concept and its significance in prokaryotic gene regulation in simple terms. What

Discuss the operon concept and its significance in prokaryotic gene regulation. Read More »

A) Describe the steps of translation, including initiation, elongation, and termination.

Introduction Translation is the process by which cells build proteins based on the information in messenger RNA (mRNA). It is a vital step in gene expression and takes place in the cytoplasm at the ribosomes. In this answer, we will break down the steps of translation: initiation, elongation, and termination. Each step ensures the correct

A) Describe the steps of translation, including initiation, elongation, and termination. Read More »

A) What are exons and introns, and how are they related to RNA splicing? B) Explain the concept of the genetic code and its role in translation.

Introduction Genes in eukaryotic cells are made up of both coding and non-coding sequences. These sequences play a vital role in the process of gene expression. In this answer, we will understand the concepts of exons and introns and how they are connected to RNA splicing. We will also explain what the genetic code is

A) What are exons and introns, and how are they related to RNA splicing? B) Explain the concept of the genetic code and its role in translation. Read More »

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