BBCCT-105 – Proteins

Explain the principle and applications of dialysis and lyophilization in protein biochemistry.

Principle and Applications of Dialysis and Lyophilization in Protein Biochemistry Introduction Protein biochemistry involves various techniques to isolate, purify, and preserve proteins for research and medical purposes. Two commonly used methods in this field are dialysis and lyophilization. Both play crucial roles in ensuring that proteins maintain their structure and functionality. 1. Dialysis Principle Dialysis […]

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Discuss the significance of peptide bonds in protein structure. Include an explanation of how peptide bonds are formed.

Significance of Peptide Bonds in Protein Structure and Their Formation What is a Peptide Bond? A peptide bond is a special type of covalent bond formed between the amino group (-NH2) of one amino acid and the carboxyl group (-COOH) of another. This bond links amino acids together to form peptides and proteins, which are

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Define amino acids and classify them based on their chemical structure and properties.

Definition and Classification of Amino Acids What are Amino Acids? Amino acids are the basic building blocks of proteins. Each amino acid consists of a central carbon atom (called the alpha carbon) bonded to four groups: An amino group (-NH2) A carboxyl group (-COOH) A hydrogen atom (H) A distinctive side chain or R group

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