BBCCT-107 – Enzymes

BBCCT-107 – Enzymes Assignment Answers Master Post

BBCCT-107 – Enzymes Assignment Answers Master Post This post contains direct links to all the answers for the BBCCT-107 Core Course in Biochemistry assignment on Enzymes. Click on each question to view the detailed, student-friendly answer. Write short note on the following terms: a) Characteristics of Enzymes b) Apoenzyme c) Coenzyme d) Turnover number Give […]

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Explain the following methods of enzyme immobilization: i) Cross Linking ii) Gel Entrapment

Enzyme Immobilization Techniques: Cross-Linking and Gel Entrapment Introduction Enzyme immobilization refers to the confinement of enzymes to a solid support or within a matrix in a way that maintains their catalytic activity. This technique offers benefits such as enzyme reusability, stability, and easier product separation. Two popular methods of immobilization include cross-linking and gel entrapment.

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Discuss application of enzymes in the following: i) Wine industry ii) Baking industry

Applications of Enzymes in Wine and Baking Industries Introduction Enzymes are biological catalysts widely used in various industrial processes due to their specificity and efficiency. In the food industry, particularly in wine and baking production, enzymes improve quality, speed up production, and enhance product stability. Their natural origin also aligns with the growing demand for

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What are multienzyme complexes? Give examples and describe their properties.

Multienzyme Complexes: Definition, Examples, and Properties Introduction Multienzyme complexes are assemblies of multiple enzymes that work together to catalyze a series of reactions in a metabolic pathway. These enzymes are physically associated into a single structure, allowing substrate channeling and efficient transfer of intermediates from one enzyme to the next. These complexes are essential for

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Write short notes on the following: i) PLP coenzymes ii) TPP

Short Notes on PLP Coenzymes and TPP i) PLP Coenzymes (Pyridoxal Phosphate) Introduction Pyridoxal phosphate (PLP) is the active coenzyme form of vitamin B6. It is essential for the metabolism of amino acids and is involved in various biochemical reactions as a coenzyme. Structure and Source PLP is derived from vitamin B6 (pyridoxine). It contains

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Explain the Strain and Distortion catalysis mechanism of enzyme. Describe reversible covalent modification of enzymes for regulating enzyme activity.

Strain and Distortion Catalysis and Reversible Covalent Modification of Enzymes Part A: Strain and Distortion Catalysis Mechanism Introduction Enzymes enhance reaction rates through various catalytic mechanisms. One such mechanism is strain or distortion catalysis, where the enzyme alters the structure of the substrate to resemble the transition state. This structural change lowers the activation energy

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Derive Michaelis-Menten equation for an uncompetitive inhibitor.

Derivation of Michaelis-Menten Equation for an Uncompetitive Inhibitor Introduction Uncompetitive inhibition is a type of enzyme inhibition where the inhibitor binds only to the enzyme-substrate (ES) complex and not to the free enzyme. This form of inhibition decreases both the maximum velocity (Vmax) and the Michaelis constant (Km) of the enzyme-catalyzed reaction, but their ratio

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Differentiate between: a) Ordered and Random mechanism for Bisubstrate reactions. b) Ligases and Lyases

Key Differences in Enzyme Mechanisms and Classes a) Ordered vs. Random Mechanism for Bisubstrate Reactions Introduction Bisubstrate reactions involve two different substrates interacting with an enzyme. The sequence in which the substrates bind to the enzyme is vital in understanding the reaction mechanism. Two common types are the Ordered mechanism and the Random mechanism. Ordered

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Explain Fischer Lock and Key Hypothesis. Derive Lineweaver Burk equation from Michaelis Menten equation.

Fischer Lock and Key Hypothesis and Derivation of Lineweaver-Burk Equation Part A: Fischer Lock and Key Hypothesis Introduction The Fischer Lock and Key Hypothesis is one of the earliest models proposed to explain the specificity of enzymes for their substrates. Introduced by Emil Fischer in 1894, this theory is a cornerstone of enzyme-substrate interaction models

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Give an overview of transition state theory of enzyme and describe the effect of pH on enzyme activity.

Overview of Transition State Theory and Effect of pH on Enzyme Activity a) Transition State Theory of Enzyme Action Transition state theory is a concept in chemistry and enzymology that explains how enzymes catalyze chemical reactions. It states that chemical reactions proceed through a high-energy intermediate state called the transition state. Enzymes function by stabilizing

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