PCR

Write short notes on any two of the following: a). Polymerase Chain Reaction (PCR) b). Hap Map Project c). Ethical principles in genetic research d). Phenylketonuria (PKU)

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a) Polymerase Chain Reaction (PCR) Polymerase Chain Reaction (PCR) is a laboratory technique used to make multiple copies of a specific segment of DNA. It was developed by Kary Mullis in 1983 and revolutionized genetic research. Steps in PCR: Denaturation: DNA is heated to separate the strands. Annealing: Short primers bind to specific sequences. Extension: […]

Write short notes on any two of the following: a). Polymerase Chain Reaction (PCR) b). Hap Map Project c). Ethical principles in genetic research d). Phenylketonuria (PKU) Read More »

Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to genetic engineering?

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Introduction The Polymerase Chain Reaction (PCR) is one of the most powerful techniques in molecular biology. It allows scientists to make millions of copies of a specific DNA segment in just a few hours. PCR has many applications in research, medicine, and genetic engineering. In this answer, we will describe the PCR process, explore its

Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to genetic engineering? Read More »

Explain the significance of synthetic oligonucleotides in molecular cloning and the steps involved in their synthesis.

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Introduction In molecular cloning, synthetic oligonucleotides play a vital role. These short sequences of nucleotides are artificially made in the lab and are used in a wide range of genetic techniques, including gene cloning, PCR, and DNA sequencing. In this answer, we will discuss the importance of synthetic oligonucleotides and explain the steps involved in

Explain the significance of synthetic oligonucleotides in molecular cloning and the steps involved in their synthesis. Read More »

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